Incharge, Pediatric Tuberculosis, HIV and Liver Clinic, B.J.Wadia Hospital for Children, Mumbai, India
Consultant in Pediatric Infectious Diseases and Pediatric Hepatology, Nanavati Hospital, Mumbai
|Address for Correspondence|
|Dr Ira Shah, 1/B Saguna, 271/B St Francis Road, Vile Parle (W), Mumbai 400056. India.|
In countries highly endemic for Tuberculosis (TB), BCG vaccine is given at birth to most children. This BCG vaccine could subsequently interfere with tuberculin skin testing (TST) when administered to screen for TB. TST remains in widespread use due to its low cost, simplicity of administration and ease of interpretation. However, results of TST in BCG vaccinated children may have to be interpreted with caution to prevent over-diagnosis of TB and unnecessary treatment with ATT. The question remains unanswered as to how long can BCG interfere with the TST results? Also what reading of TST should be taken as positive in BCG vaccinated children to determine exposure to TB.
Duration of BCG effect on TST result :
A recent study in Madrid Spain done in immigrants or adopted children from countries that give BCG at birth found that under 3 years of age, BCG does interfere with and may cause a false-positive TST result. (1) A meta-analysis by Farhat et al which included 24 studies involving 240,203 subjects who had received BCG-vaccination as infants, it was found that 20,406 (8.5%) had a TST of 10+ mm attributable to BCG, but only 56/5639 (1%) were TST-positive if tested > or =10 years after BCG. In 12 studies of 12,728 subjects vaccinated after their first birthday, 5314 (41.8%) had a false-positive TST of 10+ mm, and 191/898 (21.2%) after 10 years. This suggests that BCG may interfere with TST till 10 years of age. (2) In those countries where the vaccine is given exclusively at birth, the BCG effect disappears after 7 years (3) and even after 4 years according to a study conducted in Canada. (4) Thus, it seems that BCG does interfere with TST result upto 4 years of age and sometimes even longer and upto 10 years of age.
Boosting effect of repeated TST testing :
Children often undergo multiple tuberculin skin tests in highly endemic countries having TB. Whether TST repeated may boost the reading of TST result seems unknown. The interpretation of serial TSTs in previously BCG-vaccinated children is complicated by our inability to differentiate among increases in the TST response resulting from improved immunologic response secondary to improved nutrition, conversion secondary to recent infection, and boosting resulting from previous BCG vaccination. (5) Conversion is defined as the development of new delayed type hypersensitivity to mycobacterial antigens after new infection with M. tuberculosis, nontuberculous mycobacteria, or BCG vaccination. Boosting is the phenomenon of an increase in TST induration upon retesting in the absence of new infection. It is believed to result from recall of waned cell-mediated immunity, akin to the anamnestic serologic response. (6) TST boosting is unlikely to occur if the interval between testing is >60 days. (7) A study in Houston, Texas postulated that in young children who have had recent BCG vaccination, the influence of boosting may be greater and longer lasting. (5). Since TST once positive usually remains positive, repeated TST results are hard to interpret. (6) Thus, TST does not discriminate between recent and remote latent tuberculosis infection (LTBI). (8)
Positive TST reading with BCG -10mm or 15mm? :
A TST result of more than 10 mm is usually taken as a positive result. (9) However studies have suggested that induration greater than or equal to 15 mm should only be considered as TB infection. (10-13) in BCG vaccinated children.
BCG vaccination can have a confounding effect on TST results especially in young children. TST boosting is unlikely to occur if the interval between testing is >60 days. Induration of 15mm or more may be considered as positive in BCG vaccinated children.
|Conflict of Interest|
Last Updated : Monday, October 1, 2012 Vol 9 Issue 10 Art #65
- Pineiro R, Mellado MJ, Cilleruelo MJ, Garcia-Ascaso M, Medina-Claros A, Garcia-Hortelano M. Tuberculin skin test in bacille Calmette-Guerin-vaccinated children: how should we interpret the results? Eur J Pediatr. 2012 Jul 1. [Epub ahead of print] [Google Scholar] [Pubmed]
- Farhat M, Greenaway C, Pai M, Menzies D. False-positive tuberculin skin tests: what is the absolute effect of BCG and non-tuberculous mycobacteria? Int J Tuberc Lung Dis. 2006; 10: 1192-204. [Google Scholar] [Pubmed]
- Chan PC, Chang LY, Wu YC, Lu CY, Kuo HS, Lee CY et al. Age-specific cut-offs for the tuberculin test to detect latent tuberculosis in BCG-vaccinated children. Int J Tuber Lung Dis 2008; 12:1401-1406 [Google Scholar] [Pubmed]
- Reid JK, Ward H, Marciniuk D, Hudson S, Smith P, Hoeppner V. The effect of neonatal bacille Calmette-Guerin vaccination on purified protein derivative skin test results in Canadian aboriginal children. Chest 2007; 131:1806-1810 [Google Scholar] [Pubmed] [CrossRef]
- Mandalakas AM, Kirchner HL, Zhu X, Yeo KT, Starke JR. Interpretation of repeat tuberculin skin testing in international adoptees: conversions or boosting. Pediatr Infect Dis J. 2008; 27: 913-919. [Google Scholar] [Pubmed] [CrossRef]
- Menzies D. Interpretation of repeated tuberculin tests. Boosting, conversion, and reversion. Am J Respir Crit Care Med. 1999;159:15-21. [Google Scholar] [Pubmed]
- Cauthen GM, Snider DE Jr, Onorato IM. Boosting of tuberculin sensitivity among Southeast Asian refugees. Am J Respir Crit Care Med. 1994;149:1597-1600. [Google Scholar] [Pubmed]
- Ferrara G, Losi M, D'Amico R, Cagarelli R, Pezzi AM, Meacci M, et al. Interferon-gamma-release assays detect recent tuberculosis re-infection in elderly contacts. Int J Immunopathol Pharmacol. 2009; 22: 669-677 [Google Scholar] [Pubmed]
- Childhood TB Sub-group. Guidance for National Tuberculosis Programmes on the management of Tuberculosis in children. Geneva: WHO; 2006. Report No. WHO/HTM/TB/2006.371, WHO/FCH/CAH/2006.7
- Grupo de Trabajo de Tuberculosis de la Sociedad Espanola de Infectologia Pediatrica. Interpretation of the tuberculin skin test in children. An Esp Pediatr 2003; 59:582-585
- Moreno Perez D, Andres Martin A, Altet Gomez N, Baquero-Artigao F, Escribano Montaner A, Gomez-Pastrana Duran D et al. Diagnosis of tuberculosis in paediatrics. An Pediatr (Barc) 2010; 72:283.e1-283.e14 [Pubmed]
- Rowland K, Guthmann R, Jamieson B, Mallory D. Clinical inquires. How should we manage a patient with a positive PPD and prior BCG vaccination? J Fam Pract 2006; 55:718-720 [Google Scholar] [Pubmed]
- Wang L, Turner MO, Elwood RK, Schulzer M, FitzGerald JM. A meta-analysis of the effect of bacille Calmette-Guerin vaccination on tuberculin skin test measurements. Thorax 2002; 57:804-809 [Google Scholar] [Pubmed] [CrossRef]
|How to Cite URL :|
|Shah I. INTERPRETATION OF TUBERCULIN SKIN TESTING IN CHILDREN VACCINATED WITH BCG. Pediatric Oncall [serial online] 2012[cited 2012 January 1];9. Art #65. Available From : http://www.pediatriconcall.com/Journal/Article/FullText.aspx?artid=517&type=J&tid=&imgid=&reportid=367&tbltype=|